162 WNT-6 INDUCES THE CHONDROGENIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS

Effects of BIO on proliferation and chondrogenic differentiation of mouse marrow-derived mesenchymal stem cells

In vitro expansion of mesenchymal stem cell (MSCs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. On the other hand, some studies have indicated that BIO (6-Bromoindirubin-3-Oxime) possesses mitogenic effects on cell culture. The objective of the present study was to examine the effect of BIO on in vitro expansion and chondrogenic di...

Chondrogenic Differentiation Of Bone Marrow-derived Mesenchymal Stem Cells Regulated By Wnt/beta-catenin Signaling Pathway

IL-17 Inhibits Chondrogenic Differentiation of Human Mesenchymal Stem Cells

OBJECTIVE Mesenchymal stem cells (MSCs) can differentiate into cells of mesenchymal lineages, such as osteoblasts and chondrocytes. Here we investigated the effects of IL-17, a key cytokine in chronic inflammation, on chondrogenic differentiation of human MSCs. METHODS Human bone marrow MSCs were pellet cultured in chondrogenic induction medium containing TGF-β3. Chondrogenic differentiation ...

The Effects of the WNT-Signaling Modulators BIO and PKF118-310 on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are multipotent cells, mainly from bone marrow, and an ideal source of cells in bone and cartilage tissue engineering. A study of the chondrogenic differentiation of MSCs is of particular interest for MSCs-based cartilage regeneration. In this study, we aimed to optimize the conditions for the chrondogenic differentiation of MSCs by regulating WNT signaling using t...

Raman Spectroscopic Characterization of Hepatic Differentiation of Mesenchymal Stem Cells

Background and Aims: Mesenchymal stem cells (MSCs) are a preferred cell source for the generation of hepatocyte-like cells in regenerative medicine. They can be isolated from different sources, including adipose tissues. The Raman spectroscopy approach was evaluated for quick and efficient identification of MSCs differentiation status and a broader perspective on cell differentiation. Material...